Objective To understand the distribution, composition ratio, the population density and seasonal variation of flies and the pathogens they could carry in the different major coastal ports in Fujian province. Methods The species, composition ratio and population density and seasonal variation of flies were investigated in the different frontier ports, such as Ningde, Fuzhou Airport, Mawei, Putian, Quanzhou and Xiamen from 2006 to 2009 years. The density(number/cage·day) was calculated by baiting cage. Statistical analysis with the software SPSS 11.51 was made to evaluate the density index and seasonal fluctuation of flies in different places. And the Aerobic plate count and Coliforms in the major flies were tested. Results A total of 190 034 flies, classified into 65 species (13 in Ningde, 40 in Fuzhou airport, 37 in Mawei, 32 in Putian, 18 in Quanzhou and 21 in Xiamen) that belong to 34 genera, 5 families, were captured from 2006 to 2009. Among them, Chrysomya megacephala (42.92% ), Musca domestica (14.93%) and Lucilia sericata (10.91%) were the dominant species. The density index was highest in rubbish heap(149.42/cage·day), followed by Sewage treatment plants (139.67/cage·day) and the palaces outside of food factories (132.18 /cage·day), with freight depot/storehouse (13.08/cage·day) being the lowest in the density. The peak density in Xiamen, the Airport and Mawei was about two months later than that in Putian, Quanzhou and Ningde. Except Ningde and Fuzhou airport, a bimodal density distribution with seasonal variation was seen in the four ports. A large number of total bacteria and coliform bacteria were detected in flies in some ports. Conclusion There are more fly species in Fuzhou Airport, Mawei and Putian. The density of flies in six coastal ports is much higher than that specified in the National Control Standard. The dominant fly species in all the coastal ports are similar. The Results of fly pathogen examination and the higher fly density reveal that there is a high potential risk of intestinal infectious diseases. Environmental remediation is the basic measure for the control of flies．

【Abstract】 Objective To explore the value of isozyme zymograms in the classification of blackflies and accumulate basic biochemical data for the study of phylogenesis. Methods EST isozymes of three blackfly species were studied by discontinuous PAGE techniques. Results Ten isozyme bands could be identified according to the electrophoresis zymograms. The differences of zymograms were significant between three blackfly species and each species had its own special zymogram. The main band among intraspecific individuals or between the larvae and pupae was alike， however， other enzyme bands were different. Conclusion EST isozyme zymograms could be used as one of the classification characteristics of blackflies.

Objective By investigating population density and breeding characters of Dengue vectors in Fuzhou Changle International Airport to prevent and control Dengue fever.Methods The species and breeding characters of vectors were investigated in September,2004,the density of the larva was calculated by Breteau index,thousand-person index and container index.While the biting rate index was used to calculate density of the adult.Results Aedes albopictus was the dominant species,the Airport waiting hall has 0.00 of thousand-person index and container index.In other airport regions such as the aviation repair plant,food-plant,the dormitories region,the restaurant,the goods-station Breteau index were 0.00,7.55,4.11,33.33,25.81 respectively,the Dahe village closest to the airport was 67.92.There were five kinds small container being main breeding place of Aedes albopictus,such as a large number old jars and crocks in Airport as well as in the village.The biting rate for Aedes albopictus/ were 0.33 and 6.22/h.m in the aviation food-plant and the Dahe village.Conclusion The density of Aedes albopictus in the Dahe village nearby airport was higher than other surveillant regions.It is important to administ the envionment near the airport to control the Dengue fever.